SD/-Leu/-Met/-Trp with Agar - Names and Identifiers
SD/-Leu/-Met/-Trp with Agar - Physico-chemical Properties
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SD/-Leu/-Met/-Trp with Agar - Introduction
SD/-Leu/-Met/-Trp with Agar is a medium used for microbiological experiments. It contains nutrients with SD/-Leu/-Met/-Trp selectivity as well as hardener agar. The following is a description of the nature, use, preparation and safety information of the medium:
Nature:
-Appearance: Solid state, agar-shaped.
-Ingredients: Contains nutrients and optional additives that can promote the growth of specific strains.
Use:
-Selective culture: SD/-Leu/-Met/-Trp with Agar is used for selective culture of Leu, Met and Trp deficient yeast strains. This defect allows only strains carrying the compensation genes associated with Leu, Met and Trp to grow on this medium.
-Mutation screening: This medium can be used to screen for mutant yeast strains with specific defects.
Preparation Method:
-According to specific requirements, dissolve an appropriate amount of SD/-Leu/-Met/-Trp medium powder in an appropriate amount of distilled water according to the formula, and add an appropriate amount of agar. Dissolve evenly by automatic or manual stirring.
-Sterilize the mixture at an appropriate temperature.
-After sterilization, pour the liquid medium into a petri dish or test tube, and gel after cooling on the surface.
Safety Information:
-Strictly follow laboratory safety procedures when preparing and using media.
-This medium is mainly used for microbiological experiments, so exposure to skin, eyes or mouth should be avoided.
-Before use, please carefully read and follow the safety instructions in the safety data sheet (MSDS) and instructions provided by the supplier.
Last Update:2024-04-09 20:49:11